Questionnaire on T7 Bacteriophage RNA Polymerase
1. What is the molecular weight of the single-subunit RNA polymerase encoded by the T7 bacteriophage?
- a) 50 kDa
- b) 75 kDa
- c) 99 kDa
- d) 120 kDa
2. During which growth phase does the T7 RNA polymerase transcribe the phage's genes in Escherichia coli?
- a) Lag phase
- b) Exponential phase
- c) Lytic growth
- d) Stationary phase
3. Which structural superfamily does the T7 RNA polymerase belong to?
- a) Helicases
- b) Ligases
- c) Polymerase superfamily
- d) Transferases
4. Name the types of enzymes that share structural similarities with T7 RNA polymerase.
- a) DNA polymerases
- b) Reverse transcriptases
- c) RNA-directed RNA polymerases
- d) All of the above
5. How does the structure of T7 RNA polymerase compare to cellular RNA polymerases?
- a) It is more complex and multi-subunit
- b) It is structurally similar and multi-subunit
- c) It is structurally unrelated and simpler
- d) It has a higher molecular weight
6. What is the molecular weight range of more complex, multi-subunit cellular RNA polymerases?
- a) 100-200 kDa
- b) 200-300 kDa
- c) 300-400 kDa
- d) >500 kDa
7. What aspect of T7 RNA polymerase's structure has facilitated the description of its mechanism?
- a) Its molecular weight
- b) Its structural complexity
- c) Its relative structural simplicity
- d) Its multi-subunit nature
8. What is revealed by the progression through T7 RNA polymerase's transcription reaction?
- a) Minimal conformational changes
- b) A series of small conformational changes
- c) A concerted series of large conformational changes
- d) No conformational changes
9. What characteristics of T7 RNA polymerase are exploited in technologies for expressing heterologous genes?
- a) High activity and low promoter specificity
- b) High activity and stringent promoter specificity
- c) Low activity and stringent promoter specificity
- d) Low activity and low promoter specificity
10. In addition to expressing heterologous genes in vivo, for what other application is T7 RNA polymerase used?
- a) DNA synthesis in vitro
- b) Protein synthesis in vitro
- c) RNA synthesis in vitro
- d) Lipid synthesis in vitro
Answer Key:
- c) 99 kDa
- c) Lytic growth
- c) Polymerase superfamily
- d) All of the above
- c) It is structurally unrelated and simpler
- d) >500 kDa
- c) Its relative structural simplicity
- c) A concerted series of large conformational changes
- b) High activity and stringent promoter specificity
- c) RNA synthesis in vitro
https://affitechbio.com/rna-elisa-kit/affirec-recombinant-t7-polymerase-50u-l-afg-crb-3147